The 2000–01 North West Counties Football League season was the 19th in the history of the North West Counties Football League, a football competition in England. Teams were divided into two divisions: Division One and Division Two.
Division One
Division One featured three new teams:
Curzon Ashton, promoted as runners-up of Division Two
Flixton, relegated from the NPL Division One
Woodley Sports, promoted as champions of Division Two
League table
Division Two
Division Two featured four new teams:
Atherton Laburnum Rovers, relegated from Division One
Bootle, relegated from Division One
Padiham, promoted as champions of the West Lancashire Football League Division One Stone Dominoes, promoted as champions of the Midland Football League