The 2001 Arizona Wildcats softball team represented the University of Arizona in the 2001 NCAA Division I softball season. The Wildcats were coached by Mike Candrea, who led his seventeenth season. The Wildcats finished with a record of 65–4. They played their home games at Rita Hillenbrand Memorial Stadium and competed in the Pacific-10 Conference, where they finished first with a 19–2 record.
The Wildcats were invited to the 2001 NCAA Division I softball tournament, where they swept the West Regional and then completed a run through the Women's College World Series to claim their sixth NCAA Women's College World Series Championship.