Qualification for the 2001 Little League World Series took place in sixteen different parts of the world during July and August 2001, with formats and number of teams varying by region.
Asia
The tournament took place in Hong Kong from July 28–August 4.
Canada
The tournament was held in Vancouver, British Columbia from August 4–11.
Caribbean
The tournament took place in Panama City, Panama from July 20–28.
Europe, Middle East and Africa
The tournament took place in Kutno, Poland from August 2–10.
Great Lakes
The tournament took place in Indianapolis, Indiana from August 4–12.
Gulf
The tournament took place in Waco, Texas from August 5–11.
Latin America
The tournament took place in Panama City, Panama from July 20–28.
Mexico
The tournament took place from July 14–20.
Mid-Atlantic
The tournament took place in Bristol, Connecticut from August 5–14.
*All games played by the New York representative, Rolando Paulino Little League, were forfeited due to the use of an ineligible player, Danny Almonte. The Mid-Atlantic Championship was retroactively awarded to the Pennsylvania representative, the State College American Little League.
Midwest
The tournament took place in Indianapolis, Indiana from August 4–12.
New England
The tournament was held in Bristol, Connecticut from August 5–14.
Northwest
The tournament was held in San Bernardino, California from August 4–14.
Pacific
The tournament took place in Hong Kong from July 29–August 3.
Southeast
The tournament took place in St. Petersburg, Florida from August 3–12.
Transatlantic
The tournament was held in Kutno, Poland from July 8–15.
West
The tournament took place in San Bernardino, California from August 4–14.
