The 2016 NCAA Division III women's basketball tournament was the 35th annual tournament hosted by the NCAA to determine the national champion of Division III women's collegiate basketball in the United States.
Thomas More defeated Tufts in the championship game, 63–51, to claim the Saints' first Division III national title. While this was the second consecutive tournament win for Thomas More, the Saints' previous title from 2015 was vacated by the NCAA Committee on Infractions and does not count for official records.
The national semifinal rounds were hosted by Capital University at the Capital University Performance Arena in Columbus, Ohio, while the national championship game was held alone at Bankers Life Fieldhouse in Indianapolis, Indiana, also the site of the finals of that year's Division I and Division II tournaments.