The 1964–65 Athenian League season was the 42nd in the history of Athenian League. The league consisted of 48 teams.
Premier Division
The division featured 3 new teams, all promoted from last season's Division One: Leatherhead (1st) Worthing (2nd) Edgware Town (3rd)
League table
Division One
The division featured 5 new teams, all promoted from last season's Division Two: Tilbury (1st) Harrow Town (2nd) Harlow Town (3rd) Hertford Town (4th) Hemel Hempstead Town (5th)
League table
Division Two
The division joined 6 new teams: Harwich & Parkeston, from Eastern Counties League Croydon Amateurs, from Spartan League Herne Bay, from Aetolian League Eastbourne United, from Metropolitan League Cheshunt, from Spartan League Rainham Town, from Metropolitan League