"He filled a glass flask half full of distilled water, in which were various animal and vegetable substances: he then closed it with a good cork, through which were passed two glass tubes, bent at right angles, the whole being air-tight: it was next placed in a sand bath, and heated until the water boiled violently. While the watery vapor was escaping by the glass tubes, the Professor fastened at each end an apparatus which chemists employ for collecting carbonic acid: that at the one end was filled with concentrated sulphuric acid, and the other with a solution of potash. By means of the boiling heat, it is to be presumed that every thing living, and all germs in the flask or in the tubes were destroyed; whilst all access was cut off by the sulphuric acid on the one side, and by the potash on the other. The apparatus was then exposed to the influence of summer light and heat; at the same time, there was placed near it an open vessel, with the same substances that had been introduced into the flask, and also after having subjected them to a boiling temperature. In order to renew constantly the air within the flask, the experimenter sucked with his mouth several times a day the open end of the apparatus, filled with the solution of potash, by which process the air entered his mouth from the flask through the caustic liquid, and the atmospheric air from without entered the flask through the sulphuric acid. The air was of course not at all altered in its composition by passing through the sulphuric acid in the flask; but all the portions of living matter, or of matter capable of becoming animated, were taken up by the sulphuric acid and destroyed. From the 28th of May until the beginning of August, Professor Schulze continued uninterruptedly the renewal of the air in the flask, without being able, by the aid of the microscope, to discover any living animal or vegetable substance; although, during the whole of the time, observations were made almost daily on the edge of the liquid; and when, at last, the Professor separated the different parts of the apparatus, he could not find in the whole liquid the slightest trace of _Infusoria_ or _Confervæ_, or of mould; but all three presented themselves in great abundance a few days after he had left the flask standing open. The vessel which he placed near the apparatus contained on the following day _Vibriones_ and _Monads_, to which were soon added larger Polygastric _Infusoria_, and afterwards _Rotifera_."--pp. 32, 33.