Daffin House is a historic home located at Hillsboro, Caroline County, Maryland, United States. It is a large, 2½-story brick structure built about 1780. Attached is a two-part, 1½-story Flemish bond brick wing built about 1760, with a dormered gable roof. It was constructed by Charles Daffin who received a patent for the land in 1784 under the name of Daffin's Farm.
Future president Andrew Jackson met Charles Dickinson here in 1796. Jackson would later kill Dickinson in a duel at Adairville, Kentucky.
Daffin House was listed on the National Register of Historic Places in 1975.