To these instructions I would add some for the amateur, who will probably deal with a comparatively small number of ova. The ova should be washed in some large vessel full of water in the manner above described. When the water is quite clear, and the ova clean, they may be caught in mid-water as they are sinking either in the hatching trays or in a cup. If caught in a cup they should be transferred with great care to the hatching trays, and spread out in a single and somewhat spare layer. They must on no account be poured into the trays from a height. While under water well-eyed ova will stand a good deal of gentle tumbling about, but if dropped into the water from even a little height the concussion is likely to kill them.
Mr. Armistead recommends glass grills rather than trays such as I have described, but I have found the trays work very well, and they are very simple and clean. Glass grills are, however, very excellent, though they necessitate a somewhat greater initial outlay than do the perforated zinc trays.
A German fish culturist has recently recommended keeping a stock of fresh-water shrimps (_Gammarus pulex_) in the hatching trays and rearing boxes. He says that the shrimps eat only the dead ova, and never touch the living ones. They also eat any vegetable or animal _débris_. I have never tried the experiment myself, and so cannot speak from experience.
Dead ova should be always removed at once, and the hatching trays should be gone over carefully once or twice a day to see if any are present in them. Dead ova are easily recognized from the fact that they become opaque and white. They are best removed with a glass tube. The thumb is placed over one end of the tube, and the other end brought directly over the dead ovum. When the thumb is removed from the end of the tube held in the hand the water will rush up into the tube, carrying with it the dead ovum. The thumb is then replaced over the end of the tube, which is lifted from the water with the ovum retained in it. This tube may also be used for removing any extraneous bodies which may get into the trays or boxes.
A form of fungus known as _Byssus_ grows upon dead ova, and it is principally for this reason that they must be removed. Livingstone Stone says of _Byssus_:--"With trout eggs in water at 40° or 50° Fahrenheit, it generally appears within forty-eight hours after the egg turns white, and often sooner, and the warmer the water the quicker it comes. It is never quite safe to leave the dead eggs over twenty-four hours in the hatching boxes. The peculiarity of _Byssus_ is that it stretches out its long, slender arms, which grow rapidly over everything within its reach. This makes it peculiarly mischievous, for it will sometimes clasp a dozen or even twenty eggs in its Briarean grasp before it is discovered, and any egg that it has seized has received its death warrant." Mr. Armistead has known it appear within twenty-four hours. _Byssus_ develops only on dead ova.