Route 241 or Highway 241 may refer to:
Canada
Manitoba Provincial Road 241
Prince Edward Island Route 241
Quebec Route 241
Costa Rica
National Route 241
Japan
Japan National Route 241
United States
U.S. Route 241 (former) U.S. Route 241 (1926) U.S. Route 241 (1930s) Alabama State Route 241 Arkansas Highway 241 California State Route 241 Florida State Road 241 (former) Georgia State Route 241 Indiana State Road 241 Kentucky Route 241 Maryland Route 241 (former) Minnesota State Highway 241 Montana Secondary Highway 241 New Mexico State Road 241 New York State Route 241 North Carolina Highway 241 Ohio State Route 241 Oregon Route 241 Pennsylvania Route 241 Tennessee State Route 241 Texas State Highway 241 (former) Texas State Highway Spur 241 Farm to Market Road 241 Utah State Route 241 Virginia State Route 241 Washington State Route 241 Wisconsin Highway 241 Wyoming Highway 241