Florida was admitted to the Union on March 3, 1845, and elects its U.S. senators to class 1 and class 3. Florida's U.S. Senate seats were declared vacant in March 1861, due to its secession from the Union. They were filled again in July 1868. The state is currently represented by Republicans Marco Rubio (serving since 2011) and Rick Scott (serving since 2019). Duncan U. Fletcher was Florida's longest-serving senator (1909–1936).
List of senators
|- style="height:2em" ! rowspan=3 | 1 | rowspan=3 align=left | David Levy Yulee | rowspan=3 | Democratic | rowspan=3 nowrap | Jul 1, 1845 –Mar 3, 1851 | rowspan=3 | Elected in 1845.Lost re-election. | rowspan=3 | 1 | | rowspan=2 | 1 | rowspan=2 | Elected in 1845.Retired. | rowspan=2 nowrap | Jul 1, 1845 –Mar 3, 1849 | rowspan=2 | Democratic | rowspan=2 align=right | James Westcott ! rowspan=2 | 1
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|- style="height:2em" | | rowspan=3 | 2 | rowspan=3 | Elected in 1848.Retired. | rowspan=3 nowrap | Mar 4, 1849 –Mar 3, 1855 | rowspan=3 | Whig | rowspan=3 align=right | Jackson Morton ! rowspan=3 | 2
|- style="height:2em" ! rowspan=5 | 2 | rowspan=5 align=left | Stephen Mallory | rowspan=5 | Democratic | rowspan=5 nowrap | Mar 4, 1851 –Jan 21, 1861 | rowspan=3 | Elected in 1851. | rowspan=3 | 2 |
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|- style="height:2em" | | rowspan=4 | 3 | rowspan=3 | Elected in 1855.Withdrew. | rowspan=3 nowrap | Mar 4, 1855 –Jan 21, 1861 | rowspan=3 | Democratic | rowspan=3 align=right | David Levy Yulee ! rowspan=3 | 3
|- style="height:2em" | rowspan=2 | Re-elected in 1857.Withdrew. | rowspan=4 | 3 |
|- style="height:2em" | rowspan=2
|- style="height:2em" | rowspan=5 colspan=3 | Vacant | rowspan=5 nowrap | Jan 21, 1861 –Jun 17, 1868 | rowspan=5 | Civil War and Reconstruction. | rowspan=6 | Civil War and Reconstruction. | rowspan=6 nowrap | Jan 21, 1861 –Jun 25, 1868 | rowspan=6 colspan=3 | Vacant
|- style="height:2em" | | rowspan=3 | 4
|- style="height:2em" | rowspan=5 | 4 |
|- style="height:2em" |
|- style="height:2em" | rowspan=3 | rowspan=5 | 5
|- style="height:2em" ! rowspan=2 | 3 | rowspan=2 align=left | Adonijah Welch | rowspan=2 | Republican | rowspan=2 nowrap | Jun 17, 1868 –Mar 3, 1869 | rowspan=2 | Elected to finish term.Retired.
|- style="height:2em" | rowspan=3 | Elected to finish term.Retired. | rowspan=3 nowrap | Jun 25, 1868 –Mar 3, 1873 | rowspan=3 | Republican | rowspan=3 align=right | Thomas W. Osborn ! rowspan=3 | 4
|- style="height:2em" ! rowspan=3 | 4 | rowspan=3 align=left | Abijah Gilbert | rowspan=3 | Republican | rowspan=3 nowrap | Mar 4, 1869 –Mar 3, 1875 | rowspan=3 | Elected in 1868 or 1869.Retired. | rowspan=3 | 5 |
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|- style="height:2em" | | rowspan=3 | 6 | rowspan=3 | Elected in 1872 or 1873.Retired. | rowspan=3 nowrap | Mar 4, 1873 –Mar 3, 1879 | rowspan=3 | Republican | rowspan=3 align=right | Simon B. Conover ! rowspan=3 | 5
|- style="height:2em" ! rowspan=6 | 5 | rowspan=6 align=left | Charles W. Jones | rowspan=6 | Democratic | rowspan=6 nowrap | Mar 4, 1875 –Mar 3, 1887 | rowspan=3 | Elected in 1875. | rowspan=3 | 6 |
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|- style="height:2em" | | rowspan=3 | 7 | rowspan=3 | Elected in 1879. | rowspan=7 nowrap | Mar 4, 1879 –Mar 3, 1891 | rowspan=7 | Democratic | rowspan=7 align=right | Wilkinson Call ! rowspan=11 | 6
|- style="height:2em" | rowspan=3 | Re-elected in 1881.Retired. | rowspan=3 | 7 |
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|- style="height:2em" | | rowspan=4 | 8 | rowspan=4 | Elected in 1885.
|- style="height:2em" | colspan=3 | Vacant | nowrap | Mar 4, 1887 –May 19, 1887 | | rowspan=5 | 8 | rowspan=2
|- style="height:2em" ! rowspan=9 | 6 | rowspan=9 align=left | Samuel Pasco | rowspan=9 | Democratic | rowspan=9 nowrap | May 19, 1887 –Apr 18, 1899 | rowspan=4 | Elected late to finish term in 1887.
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|- style="height:2em" | rowspan=2 | rowspan=4 | 9 | Legislature failed to elect. | nowrap | Mar 4, 1891 –May 26, 1891 | colspan=2 | Vacant
|- style="height:2em" | rowspan=3 | Elected late in 1891.Retired. | rowspan=3 nowrap | May 26, 1891 –Mar 3, 1897 | rowspan=3 | Democratic | rowspan=3 align=right | Wilkinson Call
|- style="height:2em" | rowspan=4 | Appointed to begin next term as legislature had failed to elect.Elected in 1893 to finish term. | rowspan=4 | 9 |
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|- style="height:2em" | rowspan=2 | rowspan=6 | 10 | Legislature failed to elect. | nowrap | Mar 4, 1897 –May 13, 1897 | colspan=3 | Vacant