The 2016 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the thirteenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Knoxville, United States between 6 and 13 November 2016.
Singles main-draw entrants
Seeds
1 Rankings are as of October 31, 2016.
Other entrants
The following players received wildcards into the singles main draw: Miķelis Lībietis Mackenzie McDonald Raymond Sarmiento Rhyne Williams
The following player entered as an alternate: Sam Barry
The following players received entry from the qualifying draw: Yuki Bhambri Liam Broady Daniel Cox Jared Hiltzik
The following player received entry as a lucky loser: Tim van Rijthoven
Champions
Singles
Michael Mmoh def. Peter Polansky, 7–5, 2–6, 6–1.
Doubles
Peter Polansky / Adil Shamasdin def. Ruben Bemelmans / Joris De Loore, 6–1, 6–3.