Rostam-e Do Rural District () is in the Central District of Rostam County, Fars province, Iran.
At the National Census of 2006, its population (as a part of the former Rostam District of Mamasani County) was 6,864 in 1,338 households. There were 7,206 inhabitants in 1,876 households at the following census of 2011, by which time the district had been separated from the county in the establishment of Rostam County. At the most recent census of 2016, the population of the rural district was 6,514 in 1,888 households. The largest of its 36 villages was Deh Now-e Sadat-e Pain, with 1,066 people.