These acute symptoms were likewise produced by an extract of 500 grams of the fresh _Aragallus lamberti_ from Arizona preserved in chloroform water (rabbit weighing 1,998 grams). An aqueous extract of 150 grams of the dried _Astragalus mollissimus_[155] from Imperial, Nebr. (1906), caused death in one hour and fifty-eight minutes in a rabbit weighing 1,530 grams, and an extract of 100 grams killed in one hour and twenty-two minutes a rabbit weighing 736 grams.
An aqueous extract of 100 grams of the dried _Astragalus bigelowii_ induced death in one hour and thirty-eight minutes, the rabbit weighing 1,502 grams.
An aqueous extract of 150 grams of _Astragalus nitidus_ collected at Woodland Park, Colo., in 1906 induced death in three hours and five minutes, the rabbit weighing 1,672 grams.
An aqueous extract of 200 grams of the dried _Astragalus bisulcatus_ caused death after several hours (at night), the rabbit weighing 2,423 grams.
In certain cases this production of acute symptoms was not entirely a question of salt action, as was shown by certain other experiments. In other cases salt action seems to be the important factor, so that the production of these acute symptoms can not always be considered characteristic.
[154] In all cases in which the plants were preserved with chloroform sealed vessels were used for shipping. The chloroform was carefully evaporated off in vacuo before feeding the extract, the evaporation requiring several hours. The plants were collected by Dr. C. Dwight Marsh, in charge of the field investigations at Hugo, Colo.
[155] All extracts from dried material were made at Washington.
_Experiment No. 6._--February 19, 1906, a large gray rabbit weighing 2,055.3 grams was fed with 60 c.c. of fluid representing the concentrated aqueous extract of 250 grams of the fresh _Astragalus mollissimus_, collected September 18, 1905, and preserved in chloroform. This rabbit was very hard to hold. The ears rested on the body. The temperature at the time of feeding, 1.30 p.m., was 102.3°F. At 2.57 p. m. the animal looked dull but resisted handling. At 3.30 p.m. it urinated. At 4.15 p.m. the temperature was 98.5°F., the pupils were about the same size as before feeding, and the animal became much duller. The next day at 12.50 p.m. the temperature was 102.4°F., and at this time the animal could be handled with greater ease. The animal ate in the morning. The same amount of extract was again fed at 1.24 p. m. At 1.35 p.m. the animal was much duller and could be turned on his back with ease. If disturbed he ran against the wall as if utterly unconscious of the obstruction. The animal had soft, liquid, brown stools and tried to lie down as much as possible. If turned on its back with the feet up it would stay so almost indefinitely. Temperature, 103.8°F.; respiration very rapid. At 2.40 p.m. the temperature was 99.8°F., and the animal died a few minutes later. After death the pupils were much contracted. The vessels of the dura covering the brain were much dilated, but the vessels inside the brain were not dilated. The stomach walls were congested and marked with numerous petechiæ and covered with mucus.