Matheson House is a historic house in Perth, Ontario, Canada. It was constructed in 1840 for Roderick Matheson, a local merchant and later a member of the Senate of Canada.
A two-storey, five-bay sandstone house of Palladian-inspired design, Matheson House is surrounded by a stone garden wall. After Roderick Matheson's death in 1873, the house was inherited by Arthur James Matheson, a lawyer and member of the Legislative Assembly of Ontario. After a series of subsequent owners, the house was acquired by the municipality and became the Perth Museum.
The house was designated a National Historic Site of Canada in 1966.