Golgin subfamily A member 8H, also known as GOLGA8H, is a protein that in Homo sapiens is encoded by the GOLGA8H gene. Function of the GOLGA8H involves a process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the Golgi apparatus.
Gene
Aliases
The most common aliases for GOLGA8H are the following:
Golgin A8 Family Member H 2 3 5 or Member H 2
Golgi Autoantigen, Golgin Subfamily A, 6-Like 11 2 3
Golgin Subfamily A Member 8H 3 4
Golgin Subfamily A Member 8-Like Protein 1 3
GOLGA6L11
Prevalence and Location
GOLGA8H, when compared to many other genes, exists in many different places that span multiple chromosomes:. NCBI lists the gene’s location on the long (q) arm on Chromosome 15 in the q13.2 region, from 30,604,030 - 30,617,827 (13,798 nt in length)
In actuality, when running the FASTA protein sequence of GOLGA8H on BLAT (the BLAST-Like Alignment Tool), it is found to exist in 85 or 87 different locations (depending on an individual’s sex chromosomes). 81 copies of the protein exist on chromosome 15, one copy each on chromosomes 7, 9,10, and 12, and two copies on the Y chromosome
Copies of GOLGA8H in Homo Sapiens
Neighborhood
It would be tedious and inefficient to list all gene neighborhoods for the 87 locations of GOLGA8H. Thus, here are surrounding genes of GOLGA8H on chromosome 15 in the q13.2 region listed on NCBI:
Gene Neighborhood of GOLGA8H on Chromosome 15 q13.2 in Homo sapiens:
Transcript
There are no isoforms of GOLGA8H.
Multiple Sequence Alignment
Paralogs
A multiple sequence alignment (MSA) of GOLGA8H and its top seven paralogs was created using Clustal Omega [1]. [Appendix A] All eight genes from the Golgin Subfamily A Member 8 group were 632 amino acids in length [1]. All 632 amino acids of GOLGA8H and its top seven paralogs were analyzed and compared using Clustal Omega were analyzed and compared in an attempt to understand what makes Golgin Subfamily A Member 8H, GOLGA8H, a distinct entity. Two amino acids make GOLGA8H unique: Valine at amino acid 32 and Cysteine at amino acid 169. For all seven paralogs, the amino acid in position 32 is Isoleucine and the amino acid in position 169 is Arginine
Protein
The predicted molecular weight of GOLGA8H, rounded down to three significant figures, is 71.3 kDa. This is a theoretical value; predicted molecular weights are merely based on the amino acids present in the protein. The theoretical isoelectric point of GOLGA8H, rounded down to one significant figure, is a pI of 8
Composition
When compared to other human proteins, GOLGA8H is semi glutamine- and glutamate-enriched. In contrast, GOLGA8H is depleted in threonine, phenylalanine, and tyrosene.
There are no charge runs, hydrophobic segments, or transmembrane domains in the GOLGA8H protein. There are 62 amino acid multiplets for the protein, which is higher than the expected range. It also has amino acid patterns with high periodicity
Motifs
There are 11 motifs present in GOLGA8H:. The single experimentally-verified motif is a glutamine-rich protein located in the 323-416 amino acid region.
Post-Translational Modifications
GOLGA8H is predicted to undergo phosphorylation at multiple locations of serine, threonine, and tyrosine throughout its structure. It is expected to undergo phosphorylation most frequently on serine amino acids. Furthermore, there is one predicted N-linked glycosylation site, which occurs at amino acid 39. The sequence for this site is NGS. N-linked glycosylation functions intrinsically and extrinsically to assist in regulating the migration patterns of cells.
Primary Sequence
The protein is 632 amino acids long. It has 19 exons and two polyadenylation signals. Its sequence only partially matches a Kozak consensus sequence.
Secondary Structure
The predicted secondary structure of GOLGA8H is composed of 81% alpha helices, 25.6% beta sheets, and 17.2% turns.
Using Phyre2, 284 residues (45% of GOLGA8H) was modeled with 97.8% confidence by the single highest scoring template. This structure shows an extremely high proportion of alpha helices:
Tertiary Structure
A predicted model for a tertiary structure of GOLGA8H was generated using I-TASSER
Transcript level regulation
Promoter
There is one promoter for the GOLGA8H gene, GXP_2235212, which is 1197 nt long. It lies from base pairs 30,603,030 to 30,604,226 on the positive strand