On the other hand, Schwann, Schroeder and Dutch, and Pasteur, have amply proved that air may be allowed to have free access to beer-wort, without exciting fermentation, if only efficient precautions are taken to prevent the entry of particles of yeast along with the air.
Thus, the truth that the fermentation of a simple solution of sugar in water depends upon the presence of yeast, rests upon an unassailable foundation; and the inquiry into the exact nature of the substance which possesses such a wonderful chemical influence becomes profoundly interesting.
The first step towards the solution of this problem was made two centuries ago by the patient and painstaking Dutch naturalist, Leeuwenhoek, who in the year 1680 wrote thus:--
"Saepissime examinavi fermnentum cerevisiae, semperque hoc ex globulis per materiam pellucidam fluitantibus, quarm cerevisiam esse censui, constare observavi: vidi etiam evidentissime, unumquemque hujus fermenti globulum denuo ex sex distinctis globulis constare, accurate eidem quantitate et formae, cui globulis sanguinis nostri, respondentibus.
"Verum talis mihi de horum origine et formatione conceptus formabam; globulis nempe ex quibus farina Tritici, Hordei, Avenae, Fagotritici, se constat aquae calore dissolvi et aquae commisceri; hac, vero aqua, quam cerevisiam vocare licet, refrigescente, multos ex minimis particulis in cerevisia coadunari, et hoc pacto efficere particulam sive globulum, quae sexta pars est globuli faecis, et iterum sex ex hisce globulis conjungi."[3]
[Footnote 3: Leeuwenhoek, _Arcana Naturae Detecta._ Ed. Nov., 1721.]
Thus Leeuwenhoek discovered that yeast consists of globules floating in a fluid; but he thought that they were merely the starchy particles of the grain from which the wort was made, rearranged. He discovered the fact that yeast had a definite structure, but not the meaning of the fact. A century and a half elapsed, and the investigation of yeast was recommenced almost simultaneously by Cagniard de la Tour in France, and by Schwann and Kützing in Germany. The French observer was the first to publish his results; and the subject received at his hands and at those of his colleague, the botanist Turpin, full and satisfactory investigation.