This is a list of railroads operating in the U.S. state of Florida.
Current railroads
Common freight carriers
Alabama and Gulf Coast Railway (AGR) AN Railway (AN) Bay Line Railroad (BAYL) CSX Transportation (CSXT) First Coast Railroad (FCRD) Florida Central Railroad (FCEN) Florida East Coast Railway (FEC) Florida Gulf & Atlantic Railroad (FGA) Florida Midland Railroad (FMID) Florida Northern Railroad (FNOR) Georgia and Florida Railway (GFRR) Jacksonville Port Terminal Railroad (JXPT) Norfolk Southern Railway (NS) through subsidiary Georgia Southern and Florida Railway (GSF) Seminole Gulf Railway (SGLR) South Central Florida Express, Inc. (SCXF)
Private freight carriers
CF Industries (CFIZ) Conrad Yelvington Distributors, Inc. (CNYX) The Mosaic Company (IMCX) Orlando Utilities Commission (OUCX) Perdue Farms (PFMX) Port Manatee Railroad (MAUP) Port of Palm Beach District (PPBD) Port of Panama City (PPC) United States Sugar Corporation (USCX)
Passenger carriers
Amtrak (AMTK) Brightline (BLFX) Florida Railroad Museum (FGCX) Gold Coast Railroad Museum (GCOX) Jacksonville Skyway Miami Metromover and Miami Metrorail Orlando & Northwestern Railway (ONWX) Seminole Gulf Railway (SGLR) Serengeti Express (located in Busch Gardens Tampa) SunRail (SRCX) Tradewinds & Atlantic Railroad TECO Line Streetcar Tri-Rail (TRCX) Walt Disney World Monorail and Walt Disney World Railroad