Ons Jabeur defeated the defending champion Belinda Bencic in a rematch of the previous year's final, 7–6(8–6), 6–4 to win the singles tennis title at the 2023 Charleston Open. Jabeur did not drop a set en route to her fourth career singles title and first of the season.
This was the first tournament on the WTA Tour since the 2018 Italian Open where both finalists contested the final in consecutive years. This would be repeated only 2 weeks later at Stuttgart.
The top four seeds reached the semifinals for the first time since 2000, and for the first time at a WTA 500 tournament since the 2012 Porsche Tennis Grand Prix - Singles.
Seeds
The top eight seeds received a bye into the second round.