This is a list of qualifying teams in the 2001 NCAA Division I men's basketball tournament. A total of 65 teams entered the tournament. Twenty-nine of the teams earned automatic bids by winning their conference tournaments. The automatic bids of the Ivy League and Pacific-10, which did not conduct a postseason tournament, went to their regular season champion. The remaining 34 teams were granted at-large bids, which were extended by the NCAA Selection Committee. All teams were seeded within their assigned region from 1 to 16, which was disclosed, while the Selection Committee seeded the entire field from 1 to 65, which was not disclosed.
Qualifying teams
Automatic bids
Automatic bids to the tournament were granted for winning a conference championship tournament, except for the automatic bid of the Ivy League and Pac-10 given to the regular season champion. Seeds listed were seeds within the conference tournaments. Runners-up in bold face were given at-large berths.