John Dudley Charlesworth (October 13, 1902 – September 28, 1962) was an American football player.
Charlesworth enrolled at Yale University and, while there, played on the Yale Bulldogs football team in 1925 and 1927. He was a consensus choice for the 1927 College Football All-America Team.
Charlesworth graduated from Yale in 1929. He was married and had a son, John D. Charlesworth, Jr. He worked as a sales executive at the Shatz Painting Company in Manhattan. In September 1962, he died from a heart attack at his home on East 77th Street after returning home from eating out.