In this year of his second great triumph Pasteur was appointed director of science studies in the Ecole Normale, from which he had graduated in 1847. Two years later the loss of his daughter by a communicable disease--typhoid fever--had a great effect on his sensitive and profound mind. Many of his opponents, it is true, found Pasteur implacable in controversy. Undoubtedly he had the courage of his convictions, and his belief that, for the sake of human welfare, right views--_his_ views won by tireless experiment--must prevail, gained him the name of a fighter. But in all the intimate relations of life his essential tenderness was manifest. Like Darwin he had a horror of inflicting pain, and always insisted, when operations on animals were necessary in the laboratory, on the use of anæsthetics (our command of which had been greatly advanced by Simpson in 1847). Emile Roux said that Pasteur's agitation at witnessing the slightest exhibition of pain would have been ludicrous if, in so great a man, it had not been touching.
A few months after his daughter's death Pasteur wrote to one of his friends: "I am pursuing as best I can these studies on fermentation, which are of great interest, connected as they are with the impenetrable mystery of life and death. I am hoping to make a decisive advance very soon, by solving without the least lack of clearness the famous question of spontaneous generation." Two years previously a scientist had claimed that animals and plants could be generated in a medium of artificial air or oxygen, from which all atmospheric air and all germs of organized bodies had been precluded. Pasteur now filtered atmospheric air through a plug of cotton or asbestos (a procedure which had been followed by others in 1854), and proved that in air thus treated no fermentation takes place. Nothing in the atmosphere causes life except the micro-organisms it contains. He even demonstrated that a putrescible fluid like blood will remain unchanged in an open vessel so constructed as to exclude atmospheric dust.
Pasteur's critics maintained that if putrefaction and fermentation be caused solely by microscopic organisms, then these must be found everywhere and in such quantities as to encumber the air. He replied that they were less numerous in some parts of the atmosphere than in others. To prove his contention he set out for Arbois with a large number of glass bulbs each half filled with a putrescible liquid. The necks of the bulbs had been drawn out and hermetically sealed after the contents had been boiled. In case the necks were broken (to be again sealed immediately), the air would rush in, and (if it held the requisite micro-organisms) furnish the conditions for putrefaction. It was found that in every trial the contents of a certain number of the bulbs always escaped alteration. Twenty were opened in the country near Arbois free from human habitations. Eight out of the twenty showed signs of putrefaction. Twenty were exposed to the air on the heights of the Jura at an altitude of eight hundred and fifty meters above sea-level; the contents of five of these subsequently putrefied. Twenty others were opened near Mont Blanc at an altitude of two thousand meters and while a wind was blowing from the Mer de Glace; in this case the contents of only one of the bulbs became putrefied.