Having studied Eastern languages at the University of Prague, he had hoped to become a missionary in India, but he soon abandoned this intention and devoted himself to the study of Slavic antiquity. In 1779 he made his appearance in criticism with a periodical which set itself the task of telling "the truth, the naked, unvarnished truth" without regard for persons. He at once attracted attention by his sharp, critical acumen. His main interest lay in the purification of the Cech language and the formation of a literary norm. In 1792 his desire to reconstruct the Slavic past took him on a long journey to the libraries of Sweden and Russia, and even to the Caucasus, where he had expected to find some indications of a Cech origin. In the same year appeared his "History of the Bohemian Language and Literature," in which he described the struggles of the Cech language against the German and Latin from the time of Hus until his day, and showed what relation it bore to the other Slavic languages. The effect of this work upon the nationalistic feeling was very great. Especially his grammar of the Cech language which he published in 1808 formed the basis for all Slavic grammars written in the first half of the nineteenth century. Dobrovský was a voluminous writer, and his scientific correspondence, lately edited by Jagic, contains an immense amount of material which throws a light upon the history of the Slavic renascence.
Dobrovský soon gained many disciples in the Slavic world. The Russians Vostokov, Kalaydovich, Stroev, and many others, the Slovenes Kopitar and Vodník were his followers, and the great Slavists Safarík and Miklosich carried on the work of philology after him. He enjoyed the friendship of German scholars and poets, Goethe, Jacob Grimm, Pertz, and others. Goethe wrote of him: "Abbé Joseph Dobrovský, the past master of critical historical science in Bohemia, this rare man who long before had followed the general study of the Slavic languages and histories with genial industry and Herodotic travels, rejoiced in reducing his gains to the study of the Bohemian people and country, and thus united with the greatest glory in science the rare reputation of a popular name. The master is visible in whatever he attempts. He everywhere grasps his subject and deftly unites the fragments into one whole."