The 2016 Samarkand Challenger was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 9 and 14 May 2016.
Singles main-draw entrants
Seeds
1 Rankings are as of May 2, 2016.
Other entrants
The following players received wildcards into the singles main draw: Sanjar Fayziev Temur Ismailov Shonigmatjon Shofayziyev Pavel Tsoy
The following players received entry from the qualifying draw: Bar Tzuf Botzer Mikhail Elgin Markos Kalovelonis Francesco Vilardo
Champions
Singles
Karen Khachanov def. Rubén Ramírez Hidalgo, 6–1, 6–7(6–8), 6–1
Doubles
Denis Matsukevich / Andrei Vasilevski def. Hsieh Cheng-peng / Yang Tsung-hua, 6–4, 5–7, [10–5]