The 1958 Florida A&M Rattlers football team was an American football team that represented Florida A&M University as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 1958 NCAA College Division football season. In their 14th season under head coach Jake Gaither, the Rattlers compiled a 7–2 record, won the SIAC championship, and outscored opponents by a total of 263 to 135. The team played its home games at Bragg Memorial Stadium in Tallahassee, Florida.
The Rattlers lost to undefeated black college national champion Prairie View A&M in the Orange Blossom Classic.
The team's statistical leaders included Leroy Hardee with 704 rushing yards, 99 receiving yards, and 52 points scored, and Lee Royster with 269 passing yards.