Route 27, or Highway 27, may refer to:
Australia
Burke Developmental Road (Queensland) Zeehan Highway (Tasmania)
Canada
Alberta Highway 27
British Columbia Highway 27
Manitoba Highway 27
Prince Edward Island Route 27
Saskatchewan Highway 27
Ontario
Ontario Highway 27
York Regional Road 27, formerly Highway 27 in York Region
Simcoe County Road 27, formerly Highway 27 in Simcoe County
Chile
Chile Route 27
Costa Rica
National Route 27
Croatia
D27 road (Croatia)
Czech Republic
part of I/27 Highway; Czech: :cz:Silnice I/27
France
A27 autoroute
Route nationale 27
Germany
Bundesautobahn 27
Bundesstraße 27
Greece
Motorway 27 (Greece) Greek National Road 27
India
National Highway 27 (India)
Iran
Road 27
Ireland
N27 road (Ireland)
Italy
Autostrada A27
Japan
Japan National Route 27
Maizuru-Wakasa Expressway
Korea, South
Suncheon–Wanju Expressway
National Route 27
Montenegro
R-27 regional road (Montenegro)
Netherlands
A27 motorway (Netherlands)
New Zealand
New Zealand State Highway 27
Norway
Norwegian County Road 27
Russia
M27 highway (Russia)
Spain
Autovía A-27
Switzerland
European route E27 (partly also in France and Italy)
United Kingdom
British A27 (Whiteparish-Pevensey) M27 (Cadnam-Portsmouth)
United States
Interstate 27
U.S. Route 27
Alabama State Route 27
Arkansas Highway 27
Arkansas Highway 27B
Arkansas Highway 27N (former) California State Route 27 County Route A27 (California) County Route J27 (California) County Route S27 (California) Connecticut Route 27 Florida State Road 27 Georgia State Route 27 Idaho State Highway 27 Illinois Route 27 (former) Iowa Highway 27 K-27 (Kansas highway) Louisiana Highway 27 Maine State Route 27 Maryland Route 27 Maryland Route 27B Maryland Route 27C Maryland Route 27D Maryland Route 27E Massachusetts Route 27 M-27 (Michigan highway) Minnesota State Highway 27 County Road 27 (Hennepin County, Minnesota) County Road 27 (Ramsey County, Minnesota) County Road 27 (Scott County, Minnesota) County Road 27 (Washington County, Minnesota) Mississippi Highway 27 Missouri Route 27 Missouri Route 27 (1922) (former) Nebraska Highway 27 Nebraska Spur 27D Nebraska Recreation Road 27B Nebraska Recreation Road 27C Nevada State Route 27 (former) New Hampshire Route 27 New Jersey Route 27 County Route 27 (Bergen County, New Jersey) County Route 27 (Monmouth County, New Jersey) County Route 27 (Ocean County, New Jersey) New Mexico State Road 27 New York State Route 27 County Route 27 (Allegany County, New York) County Route 27 (Cattaraugus County, New York) County Route 27 (Clinton County, New York) County Route 27 (Columbia County, New York) County Route 27 (Dutchess County, New York) County Route 27 (Erie County, New York) County Route 27 (Greene County, New York) County Route 27 (Lewis County, New York) County Route 27 (Livingston County, New York) County Route 27B (Livingston County, New York) County Route 27 (Nassau County, New York) County Route 27 (Ontario County, New York) County Route 27 (Oswego County, New York) County Route 27 (Rensselaer County, New York) County Route 27 (Rockland County, New York) County Route 27 (Schoharie County, New York) County Route 27 (Schuyler County, New York) County Route 27 (St. Lawrence County, New York) County Route 27B (Suffolk County, New York) (former) County Route 27 (Ulster County, New York) County Route 27 (Washington County, New York) County Route 27 (Westchester County, New York) County Route 27 (Wyoming County, New York) County Route 27 (Yates County, New York) North Carolina Highway 27 North Dakota Highway 27 Ohio State Route 27 (1923-1927) (former) Oklahoma State Highway 27 Oregon Route 27 Pennsylvania Route 27 South Carolina Highway 27 South Dakota Highway 27 Tennessee State Route 27 Texas State Highway 27 Farm to Market Road 27 Texas Park Road 27 Utah State Route 27 Virginia State Route 27 Virginia State Route 27 (1918-1933) (former) Virginia State Route 27 (1940–1953) (former) Washington State Route 27 West Virginia Route 27 Wisconsin Highway 27