The 2021 Macha Lake Open was a professional women's tennis tournament played on outdoor clay courts. It was the nineteenth edition of the tournament which was part of the 2021 ITF Women's World Tennis Tour. It took place in Staré Splavy, Czech Republic between 14 and 20 June 2021.
Singles main-draw entrants
Seeds
1 Rankings are as of 31 May 2021.
Other entrants
The following players received wildcards into the singles main draw: Nikola Bartůňková Sára Bejlek Linda Nosková Darja Viďmanová
The following player received entry using a protected ranking: Destanee Aiava Alexandra Dulgheru
The following players received entry from the qualifying draw: Irene Burillo Escorihuela Miriam Kolodziejová Johana Marková İpek Öz Andreea Prisăcariu Dominika Šalková Tereza Smitková Kimberley Zimmermann
Champions
Singles
Zheng Qinwen def. Aleksandra Krunić, 7–6(7–5), 6–3
Doubles
Valentini Grammatikopoulou / Richèl Hogenkamp def. Amina Anshba / Anastasia Dețiuc, 6–3, 6–4