The Skanderborg–Skjern railway line () is a long standard gauge single track railway line in Denmark which runs between the cities of Skanderborg and Skjern in Central Jutland, Denmark.
The railway opened in sections. The section from Skanderborg to Silkeborg opened in 1871, the section from Silkeborg to Herning in 1877, and the section from Herning to Skjern in 1881. The line is owned and maintained by Rail Net Denmark and served with passenger trains by the railway company Arriva.