The Hefei–Wuhan railway () is a high-speed railway in Chinese provinces of Anhui and Hubei, with trains running from Anhui's capital Hefei to Hubei's capital Wuhan. The railway opened on 31 December 2008, high-speed services started on April 1, 2009. at and has been used by Shanghai–Nanjing–Hefei–Wuhan express trains since then.
As of July 2010, scheduling systems showed nine daily D-series express trains running in each direction between Hefei and Wuhan's three train stations (Wuhan, Hankou, and Wuchang), making the trip in 2 hours to 2 hrs 40 min. Another six Shanghai-Wuhan D-series trains passed this section without stopping in Hefei.
As of September 2013 the number of Daily departures each way is over 20 in order to increase train speed the line was upgraded to .
This railway is one of the sections of the important east-west route known as the Shanghai–Wuhan–Chengdu High-Speed Railway (Shanghai–Hankou–Chengdu).
The railway uses tunnels when crossing the Dabie Mountains on the Anhui–Hubei border.